Product Detail

Development and evaluation of a rapid phenotypic antimicrobial susceptibility test for clinical use

Title: • All the required reagents of the diagnostic assay were produced and analytically validated for assay performance. ppGpp sensor was not taken forward since it did not give consistent results. • Testing of various micro-well design for assay, select components of assay • Quality testing of the assay • Developed a self-contained kit • Identify isolates from existing clinical isolates in the clinical microbiology lab of partner 100 completed • Standard microbiological testing of clinical isolates 100 completed • Evaluation and analysis of comparative performance for non-inferiority of the new diagnostic test • We aimed to develop the prototype rapid diagnostic kit with all the necessary components and evaluate this kit with clinical isolates of Klebsiella pneumonia and uropathogenic E. coli in this proposal. • • This assay can be adapted to any secondary or tertiary level facility for guided clinical decision making for UTI and sepsis management. We hope that this approach will result in a paradigm shift in diagnostic microbiology and will have a significant impact in fighting AMR, as rapid and right diagnosis of resistance/susceptibility is the first major step towards reducing emergence and spread of AMR. A real time pilot clinical evaluation is going on in collaboration with AIIMS-Delhi and will lead to further improvement and optimisation of the product in another two years time.

Innovator: Translational Health Science and Technology Institute THSTI

USP: • This assay can be adapted to any secondary or tertiary level facility for guided clinical decision making for UTI and sepsis management. We hope that this approach will result in a paradigm shift in diagnostic microbiology and will have a significant impact in fighting AMR, as rapid and right diagnosis of resistance/susceptibility is the first major step towards reducing emergence and spread of AMR.